C4B antibody

Catalog No. ABIN6936545

Product Details anti-C4B Antibody

Target: C4B (C4b) (Complement Component C4b (C4b))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C4B antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 Molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Complement 4d protein

Clone: SPM545

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunofluorescence (1:50-1:100), Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Rejected Renal Transplant Tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for C4B

Target: C4B (C4b) (Complement Component C4b (C4b))

Alternative Name: C4B (C4b Products)

Synonyms: C4B1 antibody, C4B12 antibody, C4B2 antibody, C4B3 antibody, C4B5 antibody, C4BD antibody, C4B_2 antibody, C4F antibody, CH antibody, CO4 antibody, CPAMD3 antibody, C4 antibody, C4A2 antibody, C4A3 antibody, C4A4 antibody, C4A6 antibody, C4AD antibody, C4S antibody, CPAMD2 antibody, RG antibody, wu:fi14a03 antibody, C4B antibody, DKFZP469I0332 antibody, Slp antibody, Ss antibody, C4-2 antibody, C4l antibody, complement C4B (Chido blood group) antibody, complement C4A (Rodgers blood group) antibody, complement component 4 antibody, complement C4-B antibody, complement component 4B (Chido blood group) antibody, complement component 4A (Rodgers blood group) antibody, C4B antibody, C4A antibody, c4 antibody, LOC462577 antibody, C4a antibody, C4b antibody

Background: Acidic Complement C4, Basic C4, C4, C4-1, C4a Anaphylatoxin, C4A, C4A2, C4A3, C4A4, C4A6, C4b, C4B1, C4B12, C4d fragment, C4F, C4S, Complement C4 Gamma Chain, Complement Component 4A, Complement Component 4B,Complement 4d (C4d) (Acute Humoral Rejection Marker)
Cellular localisation: Intracytoplasmic vacuoles of endothelial cells and Secreted

Molecular Weight: 192kDa (predicted)

Gene ID: 720, 721, 534847

Pathways: Complement System