Recombinant CA9 antibody

Catalog No. ABIN6936559

The Rabbit Monoclonal anti-CA9 antibody (Clone CA9-2993R) (ABIN6936559) specifically detects CA9 in IHC. The antibody is reactive with Human and Horse samples.

Quick Overview for Recombinant CA9 antibody (ABIN6936559)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Antibody Type: Recombinant Antibody

Reactivity: Human, Horse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CA9 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: CA9-2993R

Product Details anti-CA9 Antibody

Purpose: Recombinant Renal Cell Carcinoma (Carbonic Anhydrase IX) Antibody

Specificity: Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Cross-Reactivity (Details): Human. Horse.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human CA9 protein

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Normal kidney or renal cell carcinoma (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: CA9

Background:

CA-IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250

Normal kidney or renal cell carcinoma (IHC).

Molecular Weight: 55kDa

Gene ID: 768, 63287

UniProt: Q16790