Cyclin A antibody

Catalog No. ABIN6936582

The Mouse Monoclonal anti-Cyclin A antibody has been validated for IHC (f). It is suitable to detect Cyclin A in samples from Human.

Quick Overview for Cyclin A antibody (ABIN6936582)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin A antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CCNA2-2333

Product Details anti-Cyclin A Antibody

Specificity: It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Full length recombinant Cyclin A2 protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K-562 and HeLa cells, Tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: CCNA2

Background: CCN1, CCNA, CCNA2, Cyclin A2,Cyclin A2 (S- & G2-phase Cyclin)
Cellular localisation: Nuclear and Cytoplasmic

Molecular Weight: 54kDa

Gene ID: 890, 58974

UniProt: P20248

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA