Cyclin B2 antibody

Catalog No. ABIN6936587

This Mouse Monoclonal antibody specifically detects Cyclin B2 in ELISA, IHC and Coat. It exhibits reactivity toward Xenopus laevis.

Quick Overview for Cyclin B2 antibody (ABIN6936587)

Target: Cyclin B2 (CCNB2)

Reactivity: Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat)

Clone: X29-2

Product Details anti-Cyclin B2 Antibody

Specificity: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

Cross-Reactivity (Details): Mouse. Rat. Human. Xenopus laevis.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length Xenopus laevis Cyclin B2 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human cervical tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Cyclin B2

Target: Cyclin B2 (CCNB2)

Alternative Name: CCNB2

Background: Ccnb2, CycB2, Cyclin B2, G2 mitotic specific cyclin B2, HsT17299, MGC108931, MGC140694,Cyclin B2
Cellular localisation: Cytoplasm.

Molecular Weight: 51kDa

Gene ID: 9133, 194698

UniProt: O95067

Pathways: Cell Division Cycle, M Phase