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CD14 antibody

CD14 Reactivity: Human FACS, IF Host: Mouse Monoclonal LPSR-927 unconjugated

Catalog No. ABIN6936599

Datasheet as PDF

Target See all CD14 Antibodies

CD14
Reactivity
224

45

30

29

28

28

25

24

22

18

15

14

10

9

8

8

8

5

2

2

2

1

1

1
Human
Host
185

58

16

3

1
Mouse
Compatible Secondaries
Clonality
203

60
Monoclonal
Conjugate
108

28

21

15

12

5

5

4

4

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
Application
185

93

56

47

42

42

20

15

14

13

13

12

7

4

4

3

3

3

3

2

1

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity

Recognizes a protein of 55 kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Cross-Reactivity (Details)

Human.

Purification

1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen

Recombinant human CD14 protein

Clone

LPSR-927

Isotype

IgG1 kappa

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CD14 Reactivity: Human IHC, ELISA Host: Mouse Monoclonal 1A3B6 unconjugated

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CD14 Reactivity: Human FACS, WB, IHC, IF, ELISA Host: Mouse Monoclonal 14G1F3 unconjugated

anti-CD14 (CD14) (AA 230-345) antibody
CD14 Reactivity: Human FACS, WB, IHC, ELISA Host: Mouse Monoclonal 1D1F8 unconjugated

anti-CD14 (CD14) (AA 25-148) antibody
CD14 Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal LPSR-2385 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP, Func Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) (AA 201-300) antibody
CD14 Reactivity: Human, Mouse, Rat, Dog, Sheep, Rabbit WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Application Notes

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.
Positive_Control: THP-1 cells and human monocytes. Lymph nodes and tonsils.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative

Azide free

Storage

-20 °C,-80 °C

Storage Comment

Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date

24 months
Target

CD14

Alternative Name

CD14 (CD14 Products)

Synonyms

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background

Lipopolysaccharide receptor, LPS-R, LPSR, Mo2, Monocyte Differentiation Antigen 14, Myeloid cell-specific leucine-rich glycoprotein,CD14 (Monocyte / Macrophage Marker)
Cellular localisation: Cell Surface

Molecular Weight

55kDa

Gene ID

929, 163867

UniProt

P08571

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades