CD14 antibody

Catalog No. ABIN6936602

This Mouse Monoclonal antibody specifically detects CD14 in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for CD14 antibody (ABIN6936602)

Target: CD14

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: LPSR-654

Product Details anti-CD14 Antibody

Purpose: CD14 (Monocyte / Macrophage Marker) Antibody

Specificity: Recognizes a protein of 55 kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human LPSR protein

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: THP-1 cells and human monocytes. Lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background:

Lipopolysaccharide receptor, LPS-R, LPSR, Mo2, Monocyte Differentiation Antigen 14, Myeloid cell-specific leucine-rich glycoprotein

THP-1 cells and human monocytes. Lymph nodes and tonsils.

Molecular Weight: 55kDa

Gene ID: 929, 163867

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades