CD70 antibody

Catalog No. ABIN6936801

Product Details anti-CD70 Antibody

Target: CD70 (CD70 Molecule (CD70))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD70 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Coating (Coat), Proliferation Assay (ProA)

Specificity: It recognizes a protein of 30 kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This MAb blocks the interaction between CD27 and CD70, and has been shown toinhibit T cell proliferation induced by dendritic cells.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human WM-1 (Waldenstrom s macroglobulinemia) cell line

Clone: BU69

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA), ,Inhibits T cell proliferation induced by dendritic cells (Order Ab without Azide), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunoprecipitation (1-2 μg/500 μg protein lysate), ,Immunohistochemistry (Frozen) (1-2 μg/mL for 30 minutes at RT),Optimal dilution for a specific application should be determined.

Positive_Control: Raji cells. Lymph node or tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD70

Target: CD70 (CD70 Molecule (CD70))

Alternative Name: CD70 (CD70 Products)

Synonyms: CD27LG antibody, Cd27l antibody, Tnfsf7 antibody, CD27L antibody, TNFSF7 antibody, CD70 antigen antibody, CD70 molecule antibody, Cd70 molecule antibody, Cd70 antibody, CD70 antibody

Background: CD27 ligand (CD27L), CD27LG, Ki24, Surface antigen CD70, Tumor necrosis factor (ligand) superfamily, member 7 (TNFSF7),CD70 / TNFSF7 (Activated T- & B-Lymphocyte Marker)
Cellular localisation: Cell Surface

Molecular Weight: 30kDa

Gene ID: 970, 501497, 715224

UniProt: P32970

Pathways: Cancer Immune Checkpoints