CFTR antibody (AA 258-385)

Catalog No. ABIN6936950

This Mouse Monoclonal antibody specifically detects CFTR in ISt and IHC (f). It exhibits reactivity toward Human.

Quick Overview for CFTR antibody (AA 258-385) (ABIN6936950)

Target: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CFTR antibody is un-conjugated

Application: Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CFTR-1785

Product Details anti-CFTR Antibody

Binding Specificity: AA 258-385

Specificity: Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 258-385) of human CFTR protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: MOLT-4 cells. Pancreas, Kidney or Placenta.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CFTR

Target: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))

Alternative Name: CFTR

Background: ABC35, ATP Binding Cassette Superfamily C Member 7 (ABCC7), cAMP-dependent chloride channel, CFTR, CFTR/MRP, Channel conductance-controlling ATPase, Cystic Fibrosis Transmembrane Conductance Regulator, MRP7, TNR CFTR,CFTR (Cystic Fibrosis Transmembrane Conductance Regulator)
Cellular localisation: Cell Surface and Cytoplasmic

Molecular Weight: 165-170kDa

Gene ID: 1080, 489786, 621460

UniProt: P13569