CHD4 antibody (C-Term)

Catalog No. ABIN6936972

The Mouse Monoclonal anti-CHD4 antibody is suitable to detect CHD4 in samples from Human and Mouse. It has been validated for WB, IF, IHC, ELISA and FACS.

Quick Overview for CHD4 antibody (C-Term) (ABIN6936972)

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CHD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 3F2-4

Product Details anti-CHD4 Antibody

Binding Specificity: C-Term

Purpose: CHD4 Antibody

Specificity: CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.

Cross-Reactivity (Details): Human. Mouse.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.

Isotype: IgG2a, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CHD4

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Alternative Name: CHD4

Background:

ATP dependent helicase CHD4, Chromodomain helicase DNA binding protein 4, Mi 2 autoantigen 218 kDa protein, Mi 2b, Mi2 beta

HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.

Molecular Weight: 238kDa

Gene ID: 1108, 162233

UniProt: Q14839