CTLA4 antibody

Catalog No. ABIN6937038

The Mouse Monoclonal anti-CTLA4 antibody is suitable to detect CTLA4 in samples from Mouse, Rat, Cow and Cat. It has been validated for WB, FACS and IF.

Quick Overview for CTLA4 antibody (ABIN6937038)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Mouse, Rat, Cow, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: L4P2F5-F10

Product Details anti-CTLA4 Antibody

Purpose: CTLA4 / CD152 (Negative Regulator of T-Cells) Antibody

Specificity: CTLA4 (CD152) is a cell surface receptor that behaves as a negative regulator of the proliferation and the effector function of T cells. It contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.The novel finding that CTLA-4 Molecule is expressed and functional on human tumor cells opens up the possibility of antitumor therapeutic intervention based on targeting this molecule.

Cross-Reactivity (Details): Cow, Cat, Mouse and Rat. Does not react with Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A synthetic peptide from CTLA4 (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat or Raji cells. Lymph node or tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA4

Background:

ALPS5, CD152, Celiac disease 3 (CELIAC3), Cytotoxic T-lymphocyte-associated antigen 4 (CTLA4), GRD4, GSE, ICOS, Insulin-dependent Diabetes Mellitus 12 (IDDM12)

Jurkat or Raji cells. Lymph node or tonsil.

Molecular Weight: 30-43kDa

Gene ID: 1493, 247824

UniProt: P16410

Pathways: Cancer Immune Checkpoints