Recombinant Cytochrome C antibody (AA 1-80)

Catalog No. ABIN6937068

Product Details anti-Cytochrome C Antibody

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 1-80

Reactivity: Pigeon

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Cross-Reactivity (Details): Human. Mouse. Rat. Horse. Dog. Pigeon. Frog. Drosophila.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c

Clone: RCYCS-1010

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or cardiac muscle. Heart tissue lysate.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: CYCS (CYCS Products)

Synonyms: CYC antibody, HCS antibody, THC4 antibody, cyct antibody, cyc antibody, hcs antibody, CYCS antibody, cytc antibody, CYCSA antibody, CG17903 antibody, Cyt C antibody, Cyt c antibody, Cyt-c2 antibody, CytC-2 antibody, Cytc antibody, Cytc-p antibody, DC4 antibody, DMc01 antibody, Dmel\\CG17903 antibody, cyt c antibody, cyt c-p antibody, cyt-c-p antibody, cyt-c2 antibody, cyt.c antibody, cytochrome c antibody, dc4 antibody, pDMc01 antibody, wu:fk52b01 antibody, zgc:86706 antibody, ENSMUSG00000058927 antibody, cytochrome c, somatic antibody, cytochrome c, somatic S homeolog antibody, Cytochrome c proximal antibody, cytochrome c, somatic b antibody, cytochrome c, somatic pseudogene antibody, cytochrome c-like antibody, cytochrome c antibody, CYCS antibody, cycs.S antibody, cycs antibody, Cycs antibody, Cyt-c-p antibody, cycsb antibody, Gm10053 antibody, LOC100724240 antibody, CYC antibody, LOC100847700 antibody, LOC101107954 antibody, LOC106829744 antibody, LOC106992390 antibody, LOC102085712 antibody

Background: CYC, CYCS, HCS, THC4,Cytochrome C (Mitochondrial Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 15kDa

Gene ID: 54205, 437060

UniProt: P99999

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity