CYP2E1 antibody (C-Term)

Catalog No. ABIN6937071

The Mouse Monoclonal anti-CYP2E1 antibody is suitable to detect CYP2E1 in samples from Human. It has been validated for IHC.

Quick Overview for CYP2E1 antibody (C-Term) (ABIN6937071)

Target: CYP2E1 (Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CYP2E1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: M12P4H2

Product Details anti-CYP2E1 Antibody

Binding Specificity: C-Term

Purpose: Cytochrome p450 Antibody

Specificity: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases, which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines, which are pre-mutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Ovalbumin-conjugated synthetic peptide HIGFGCIPPR (C-terminal sequence)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2 cells. Liver.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CYP2E1

Target: CYP2E1 (Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1))

Alternative Name: CYP2E1

Background:

4-nitrophenol 2-hydroxylase, CPE1, CYP2Ecytochrome P450 2E1, CYPIIE1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 , Cytochrome P450-J, EC 1.14.13, EC 1.14.13.n7, EC 1.14.14.1, flavoprotein-linked monooxygenase, microsomal monooxygenase, P450C2E, P450-J, xenobiotic monooxygenase

HepG2 cells. Liver.

Molecular Weight: ~50-55kDa

Gene ID: 1571, 12907

UniProt: P05181