EGLN1 antibody (AA 1-24)

Catalog No. ABIN6937142

This Mouse Monoclonal antibody specifically detects EGLN1 in WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for EGLN1 antibody (AA 1-24) (ABIN6937142)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 366G-76-3

Product Details anti-EGLN1 Antibody

Binding Specificity: AA 1-24

Specificity: 366G/76/3 recognises human prolyl hydroxylase 2 (PHD2), a 46 kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.

Cross-Reactivity (Details): Human. Immunogen sequence has 83 % identity with Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Residues 1-24 of PHD2

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF7 cells. Brain or adrenal gland.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: EGLN1

Background: Chromosome 1 Open Reading Frame 12 (C1ORF12), DKFZp761F179, ECYT3, Egl 9 family hypoxia inducible factor 1, Egl nine homolog 1, Egln1, HIF Prolyl Hydroxylase 2 (HIF PH2), HIFPH2, HPH2, Hypoxia inducible factor prolyl hydroxylase 2, ORF13, P4H2, PhD2, PNAS 118, PNAS 137, Prolyl hydroxylase domain-containing protein 2, SM20, Zinc finger MYND domain containing protein 6 (ZMYND6),EGLN1 / PHD2
Cellular localisation: Cytoplasm.

Molecular Weight: 46kDa

Gene ID: 54583, 444450

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effect