ENAH antibody (AA 485-589)

Catalog No. ABIN6937147

The Mouse Monoclonal anti-ENAH antibody (Clone ENAH-1988) (ABIN6937147) specifically detects ENAH in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for ENAH antibody (AA 485-589) (ABIN6937147)

Target: ENAH (Enabled Homolog (ENAH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENAH antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: ENAH-1988

Product Details anti-ENAH Antibody

Binding Specificity: AA 485-589

Purpose: ENAH / MENA (Actin Regulator) Antibody

Specificity: The Wiskott-Aldrich syndrome (WAS) is characterized by thrombocytopenia, eczema, defects in cell-mediated and humoral immunity and a propensity for lymphoproliferative diseases. The syndrome is the result of a mutation in the gene encoding a proline-rich protein termed WASP. WASP is a downstream effector of Cdc42 and has been implicated in actin polymerization and cyto- skeletal organization. Distantly related proteins, VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), are involved in the regulation of cytoskeletal dynamics. Both Mena and VASP accumulate at focal adhesions. Mena is highly expressed in the developing nervous system and may be involved in growth cone motility and axon guidance.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human MENA protein (around aa 485-589) (exact sequence is proprietary)

Isotype: IgG2c, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA),Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Uterus.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ENAH

Target: ENAH (Enabled Homolog (ENAH))

Alternative Name: ENAH

Background:

ENA, ENAH, MENA, NDPP1

MCF-7 cells. Uterus.

Molecular Weight: 80/88/140kDa

Gene ID: 55740, 497893

UniProt: Q8N8S7