Endoglin antibody (AA 74-251)

Catalog No. ABIN6937148

This Mouse Monoclonal antibody specifically detects Endoglin in WB, IHC and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Endoglin antibody (AA 74-251) (ABIN6937148)

Target: Endoglin (ENG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Endoglin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ENG-3269

Product Details anti-Endoglin Antibody

Binding Specificity: AA 74-251

Specificity: CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. CD105 is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for Prostate Adenocarcinoma, and cancers of the lung, stomach, breast, and brain.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Endoglin protein fragment (aa74-251) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, A431 or HL-60 cell lysates (WB). Human tonsil or placenta (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: ENG

Background: CD105, END, Endoglin, Eng, HHT1, S-endoglin,Endoglin / CD105 (Angiogenesis Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 84kDa

Gene ID: 2022, 76753

UniProt: P17813