FBXO5 antibody (AA 1-250)

Catalog No. ABIN6937217

This Mouse Monoclonal antibody specifically detects FBXO5 in WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for FBXO5 antibody (AA 1-250) (ABIN6937217)

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FBXO5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: EMI1-1176

Product Details anti-FBXO5 Antibody

Binding Specificity: AA 1-250

Specificity: It recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL for 60 minutes at RT),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, 293 or HepG2 cells. Ovarian carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for FBXO5

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Alternative Name: FBXO5

Background: Early Mitotic Inhibitor-1 (EMI1), FBX5, Fbxo31,EMI1 (Early Mitotic Inhibitor-1)
Cellular localisation: Nuclear

Molecular Weight: 56kDa

Gene ID: 26271, 520506

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases