Recombinant GFAP antibody

Catalog No. ABIN6937313

This Rabbit Monoclonal antibody specifically detects GFAP in WB, IHC, IF and FACS. It exhibits reactivity toward Human, Rat, Mouse, Cow, Pig, Rabbit, Chicken, Guinea Pig, Dog and Hamster.

Quick Overview for Recombinant GFAP antibody (ABIN6937313)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse, Cow, Pig, Rabbit, Chicken, Guinea Pig, Dog, Hamster

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: ASTRO-1974R

Product Details anti-GFAP Antibody

Purpose: Recombinant GFAP (Astrocyte & Neural Stem Cell Marker) Antibody

Specificity: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Cross-Reactivity (Details): Human. Mouse. Rat. Cow. Pig. Rabbit. Chicken. Dog

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length GFAP protein

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells),Immunofluorescence (2-3 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background:

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

T98G cells (FACS). Human Brain, Mouse Brain, Rat Brain, Hamster Brain or Guinea pig Brain (WB). Brain or Astrocytoma (IHC).

Molecular Weight: ~50kDa

Gene ID: 2670, 514227

UniProt: P14136