HLA-DRA antibody

Catalog No. ABIN6937413

The Mouse Monoclonal anti-HLA-DRA antibody has been validated for FACS and IF. It is suitable to detect HLA-DRA in samples from Human.

Quick Overview for HLA-DRA antibody (ABIN6937413)

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DRA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 169-1B5-2

Product Details anti-HLA-DRA Antibody

Specificity: This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen.It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Cross-Reactivity (Details): Human. Cow. Cat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human peripheral blood leukocytes

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for HLA-DRA

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Alternative Name: HLA-DRA

Background: DR alpha chain, HLA class II histocompatibility antigen, HLA class II histocompatibility antigen DR alpha chain, HLA DRA, HLA DRA1, HLA-DRA, HLADRA1, Major histocompatibility complex class II DR alpha, MHC cell surface glycoprotein, MHC class II antigen DRA,HLA-DRA (MHC II)
Cellular localisation: Cell surface

Molecular Weight: 36kDa (α, chain) and 27kDa (β, chain)

Gene ID: 3122, 520048

UniProt: P01903

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Human Leukocyte Antigen (HLA) in Adaptive Immune Response