IGHG antibody

Catalog No. ABIN6937497

The Mouse Monoclonal anti-IGHG antibody is suitable to detect IGHG in samples from Human. It has been validated for IHC, FACS, IF and WB.

Quick Overview for IGHG antibody (ABIN6937497)

Target: IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGHG antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Clone: IG217

Product Details anti-IGHG Antibody

Purpose: IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Antibody

Specificity: Recognizes a protein of 75 kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified human Ig Gamma Chain

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin/paraffin) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IGHG

Target: IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))

Alternative Name: IGHG

Background:

G1m Marker, G2m Marker, G3m Marker, G4m Marker, HDC, Heavy Chain Disease Protein, Human Immunglobulin G, Ig gamma1/2/3/4 Chain C Region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobulin Heavy Constant 1/2/3/4

293T, Raji or hPBL cells. Tonsil or Spleen. Human Serum.

Molecular Weight: 75kDa

Gene ID: 3500, 3501, 3502

UniProt: P01857, P01859, P01860, P01861