IGHM antibody

Catalog No. ABIN6937515

The Mouse Monoclonal anti-IGHM antibody is suitable to detect IGHM in samples from Human. It has been validated for IHC and WB.

Quick Overview for IGHM antibody (ABIN6937515)

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGHM antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: IM260

Product Details anti-IGHM Antibody

Purpose: IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Antibody

Specificity: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant heavy chain of human IgM

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Raji or hPBL cells. Human tonsil or spleen (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IGHM

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Alternative Name: IGHM

Background:

AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region

293T, Raji or hPBL cells. Human tonsil or spleen (IHC).

Molecular Weight: 50-75kDa

Gene ID: 3507, 510635

UniProt: P01871