C-JUN antibody

Catalog No. ABIN6937611

The Mouse Monoclonal anti-C-JUN antibody has been validated for WB, IF, FACS and IHC (f). It is suitable to detect C-JUN in samples from Human.

Quick Overview for C-JUN antibody (ABIN6937611)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C-JUN antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C-J 4C4-1

Product Details anti-C-JUN Antibody

Specificity: This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Cross-Reactivity (Details): Human. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: c-Jun protein phosphorylated at T91 and T93

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HEK293, HeLA or A-431 cells, Human normal lung or bladder tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: JUN

Background: Activator protein 1(API), cJun, Enhancer Binding Protein AP1, Jun Activation Domain Binding Protein, Jun proto oncogene, Transcription Factor AP1, V jun avian sarcoma virus 17 oncogene homolog, VV jun sarcoma virus 17 oncogene homolog, V-jun avian sarcoma virus 17 oncogene homolog, vJun Avian Sarcoma Virus 17 Oncogene Homolog,Phospho C-Jun (Thr91, Thr93)
Cellular localisation: Nucleus.

Molecular Weight: 39kDa

Gene ID: 3725, 696684

UniProt: P05412

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins