Cytokeratin 13 antibody

Catalog No. ABIN6937655

This Mouse Monoclonal antibody specifically detects Cytokeratin 13 in IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Cytokeratin 13 antibody (ABIN6937655)

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 13 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: KRT13-2659

Product Details anti-Cytokeratin 13 Antibody

Purpose: Cytokeratin 13 (Non-Keratinized Squamous Epithelial Marker) Antibody

Specificity: Cytokeratin 13 (KRT13) is the major acidic keratin, which together with KRT4, its basic partner, is expressed in the suprabasal layers of non-cornified stratified epithelia including tongue mucosa, esophagus, anal canal epithelium, tracheal epithelium, uterine cervix, and urothelium. Defects in the KRT13 gene are a cause of white sponge nevus of cannon (WSN), a rare autosomal dominant disorder, which predominantly affects non-cornified stratified squamous epithelia and is characterized by the presence of soft, white and spongy plaques in the oral mucosa. KRT13 has been used as a marker for non-keratinized squamous epithelium. It is also expressed in various squamous metaplasia, but it is down regulated in squamous dysplasia and squamous carcinoma.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human KRT13 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (1-2 μg per million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or A431 cells. Skin or squamous cell carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cytokeratin 13

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Alternative Name: KRT13

Background:

CK13, Cytokeratin-13, Keratin Type I Cytoskeletal 13, Keratin-13, KRT13, Type I Cytoskeletal 13, WSN2

HeLa or A431 cells. Skin or squamous cell carcinoma.

Molecular Weight: 52kDa

Gene ID: 3860, 654550

UniProt: P13646