Recombinant Cytokeratin 19 antibody

Catalog No. ABIN6937691

This Mouse Monoclonal antibody specifically detects Cytokeratin 19 in WB, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant Cytokeratin 19 antibody (ABIN6937691)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: RKRT19-799

Product Details anti-Cytokeratin 19 Antibody

Purpose: Recombinant Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) Antibody

Specificity: This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40 kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

No Cross-Reactivity: Ferret, Rat (Rattus)

Cross-Reactivity (Details): Human. Does not react with Rat or Ferret.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant human full-length KRT19 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HeLa oe Hep-G2 cells. Human breast cancer tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: KRT19

Background:

k19, k1cs, Keratin 19 Keratin Type i 40kD, krt19

HeLa, Hep-G2 cells. Human breast cancer tissue., MCF-7. Human Skin.

Molecular Weight: 40kDa

Gene ID: 3880, 654568

UniProt: P08727