KRT8, KRT18 antibody
-
- Target
- KRT8, KRT18
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
-
Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
- No Cross-Reactivity
- Chicken, Donkey, Ferret, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis
- Cross-Reactivity (Details)
- Human. Monkey. Cow. Sheep. Pig. Does not react with Mouse, Rat, Ferret, Rabbit, donkey, Chicken or xenopus laevis.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Cytoskeleton preparation from HeLa cells
- Clone
- C-51
- Isotype
- IgG1 kappa
-
-
- Application Notes
-
Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.
- Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
-
- Target
- KRT8, KRT18
- Alternative Name
- KRT8 & KRT18
- Background
-
CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18,Cytokeratin 8/18
Cellular localisation: Cytoplasmic - Molecular Weight
- 52.5kDa (CK8), 45kDa (CK18)
- Gene ID
- 3856, 3875, 533782
- UniProt
- P05787, P05783
-