KRT8, KRT18 antibody

Catalog No. ABIN6937758

The Mouse Monoclonal anti-KRT8, KRT18 antibody is suitable to detect KRT8, KRT18 in samples from Human, Cow, Monkey, Pig and Sheep. It has been validated for IHC, WB, FACS and IF.

Quick Overview for KRT8, KRT18 antibody (ABIN6937758)

Target: KRT8, KRT18

Reactivity: Human, Cow, Monkey, Pig, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: C-51

Product Details

Purpose: Cytokeratin 8/18 Antibody

Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

No Cross-Reactivity: Chicken, Donkey, Ferret, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis

Cross-Reactivity (Details): Human. Monkey. Cow. Sheep. Pig. Does not react with Mouse, Rat, Ferret, Rabbit, donkey, Chicken or xenopus laevis.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Cytoskeleton preparation from HeLa cells

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: KRT8, KRT18

Alternative Name: KRT8 & KRT18

Background:

CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18

Colon, lung or breast carcinoma., MCF-7 or A431 cells. Skin

Molecular Weight: 52.5kDa (CK8), 45kDa (CK18)

Gene ID: 3856, 3875, 533782

UniProt: P05787, P05783