LIG1 antibody

Catalog No. ABIN6937786

This anti-LIG1 antibody is a Mouse Monoclonal antibody detecting LIG1 in WB, ELISA and IP. Suitable for Cow.

Quick Overview for LIG1 antibody (ABIN6937786)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 1A9

Product Details anti-LIG1 Antibody

Specificity: DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.

Cross-Reactivity (Details): Human. Mouse. Rat. Bovine.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Full-length native cow LIG1 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ELISA, Immunoprecipitation,Optimal dilution for a specific application should be determined.

Positive_Control: MOLT-4, PC-3 or Jurkat cell lysates.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: LIG1

Background: DNA ligase 1, DNA ligase I, DNLI1_HUMAN, LIG 1, lig1, Ligase I DNA ATP dependent, MGC117397, MGC130025, Polydeoxyribonucleotide synthase [ATP] 1,DNA Ligase 1
Cellular localisation: Nucleus.

Molecular Weight: 133kDa

Gene ID: 3978, 1770

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA