Recombinant MCM7 antibody (AA 195-319)

Catalog No. ABIN6937823

This Mouse Monoclonal antibody specifically detects MCM7 in WB and IHC. It exhibits reactivity toward Human and Dog.

Quick Overview for Recombinant MCM7 antibody (AA 195-319) (ABIN6937823)

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RMCM7-1468

Product Details anti-MCM7 Antibody

Binding Specificity: AA 195-319

Purpose: Recombinant MCM7 (Proliferation Marker) Antibody

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

Cross-Reactivity (Details): Human. Dog

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant human MCM7 protein fragment (aa195-319) (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HEK-293, A549, SKBr3, HeP2, Raji or Ramos cells. Tonsil (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Alternative Name: MCM7

Background:

CDC47, DNA replication licensing factor MCM7, MCM7 mini chromosome maintenance deficient 7, Mini chromosome Maintenance 7, Mini chromosome maintenance protein 7, P1.1-MCM3, P1CDC47, P85MCM, PNAS146

A-431, A549, HEK-293, HeP2, MCF-7, Raji or Ramos cells. Tonsil (IHC)., SKBR3, Skin.

Molecular Weight: 88kDa

Gene ID: 4176, 438720

UniProt: P33993

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA