MMP2 antibody (AA 444-575)

Catalog No. ABIN6937878

The Mouse Monoclonal anti-MMP2 antibody has been validated for WB. It is suitable to detect MMP2 in samples from Human.

Quick Overview for MMP2 antibody (AA 444-575) (ABIN6937878)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MMP2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: MMP2-1501

Product Details anti-MMP2 Antibody

Binding Specificity: AA 444-575

Specificity: It recognizes a protein of 72 kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human MMP2 protein fragment (aa 444-575) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL) Optimal dilution for a specific application should be determined.

Positive_Control: U-138 MG or U-87 MG cells. Placenta or Colon Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: MMP2

Background: 72kD type IV collagenase, CLG4A, Collagenase Type 4 alpha, Collagenase type IV A, Gelatinase A, Gelatinase alpha, Gelatinase neutrophil, Matrix metalloproteinase-2, MMP2, MONA, Neutrophil gelatinase, PEX, TBE-1,MMP2 / Collagenase Type IV A (Tumor Metastasis Marker)
Cellular localisation: Cytoplasmic and Cell Surface

Molecular Weight: 72kDa (Pro), 63kDa (cleaved)

Gene ID: 4313, 513617

UniProt: P08253

Pathways: Activation of Innate immune Response