MTAP antibody (AA 97-196)
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- Target See all MTAP Antibodies
- MTAP (Methylthioadenosine phosphorylase (MTAP))
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Binding Specificity
- AA 97-196
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MTAP antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary)
- Clone
- MTAP-1813
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product MTAP Primary Antibody
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- Application Notes
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HepG2, A431, HeLa, HAP1 or MCF-7 cells. Kidney.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- MTAP (Methylthioadenosine phosphorylase (MTAP))
- Alternative Name
- MTAP (MTAP Products)
- Synonyms
- BDMF antibody, DMSFH antibody, DMSMFH antibody, LGMBF antibody, MSAP antibody, c86fus antibody, Cc1-6 antibody, 1300019I21Rik antibody, MTAPase antibody, zgc:66012 antibody, mtap antibody, methylthioadenosine phosphorylase antibody, 5'-methylthioadenosine phosphorylase antibody, 6-oxopurine nucleoside phosphorylase antibody, methylthioadenosine phosphorylase L homeolog antibody, MTAP antibody, Mtap antibody, mtaP antibody, MMAH_RS04260 antibody, TAGG_RS05050 antibody, Toce_0364 antibody, VDIS_RS03165 antibody, MFER_RS02485 antibody, Intca_0886 antibody, DESMU_RS05370 antibody, Dester_1490 antibody, ARCVE_RS05445 antibody, Mahau_1873 antibody, MZHIL_RS01440 antibody, mtap antibody, mtap.L antibody
- Background
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BDMF, DMSFH, DMSMFH, Epididymis luminal protein 249, HEL249, LGMBF, MeSAdo phosphorylase, Methylthioadenosine phosphorylase, MSAP, MTA phosphorylase, MTAP, MTAPase, S-methyl-5''-thioadenosine phosphorylase,MTAP (Tumor Suppressor Marker)
Cellular localisation: Cytoplasmic - Molecular Weight
- 31kDa
- Gene ID
- 4507, 193268
- UniProt
- Q13126
- Pathways
- Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process
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