Recombinant NGFR antibody (AA 281-421)

Catalog No. ABIN6938046

The Mouse Monoclonal anti-NGFR antibody has been validated for WB, IHC (f) and Diff. It is suitable to detect NGFR in samples from Human.

Quick Overview for Recombinant NGFR antibody (AA 281-421) (ABIN6938046)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Differentiation (Diff)

Clone: RNGFR-1965

Product Details anti-NGFR Antibody

Binding Specificity: AA 281-421

Specificity: It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human p75 NGFR protein fragment (around aa 281-421) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Neuronal axons, Schwann cells and perineural cells of peripheral nerves or tumors of nerve sheath differentiation e. G. Schwannoma, Neurofibroma. Soma and axons of sensory neurons and ganglionic satellite cells. Melanomas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR

Background: CD271, Gp80-LNGFR, Low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, Nerve growth factor receptor (NGFR), p75 ICD, p75 Neurotrophin receptor, Tumor necrosis factor receptor superfamily member 16 (TNFRSF16),NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker)
Cellular localisation: Cell surface and cytoplasmic

Molecular Weight: 75kDa

Gene ID: 4804, 415768, 681726

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding