NADPH Oxidase 4 antibody

Catalog No. ABIN6938080

This anti-NADPH Oxidase 4 antibody is a Mouse Monoclonal antibody detecting NADPH Oxidase 4 in WB, ELISA and Coat. Suitable for Human.

Quick Overview for NADPH Oxidase 4 antibody (ABIN6938080)

Target: NADPH Oxidase 4 (NOX4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NADPH Oxidase 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Coating (Coat)

Clone: NOX4-1245

Product Details anti-NADPH Oxidase 4 Antibody

Specificity: The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. NOX4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. It is also expressed in fetal tissues, placenta, glioblastoma and vascular cells.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human NOX4 protein

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA)Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: PC-12, JAR cells. Kidney.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for NADPH Oxidase 4

Target: NADPH Oxidase 4 (NOX4)

Alternative Name: NOX4

Background: Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, KOX-1, NADPH oxidase 4, Nox4, Renal NAD(P)H-oxidase, RENOX,NOX4 / NADPH Oxidase 4
Cellular localisation: Cytoplasm and Cell Surface

Molecular Weight: 70kDa

Gene ID: 50507, 371036

UniProt: Q9NPH5

Pathways: Carbohydrate Homeostasis, Smooth Muscle Cell Migration