PCNA antibody

Catalog No. ABIN6938132

This Mouse Monoclonal antibody specifically detects PCNA in WB and IHC (f). It exhibits reactivity toward Rat.

Quick Overview for PCNA antibody (ABIN6938132)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: PC5

Product Details anti-PCNA Antibody

Specificity: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Cross-Reactivity (Details): Human. Mouse. Rat. Yeast. Dog

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Rat PCNA/protein A fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Tonsil or reactive lymph node. MOLT-4, HePG2, THP-1, A431, A549, SKBr3, HEK293 and U937.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein,PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker)
Cellular localisation: Predominantly nuclear, some cytoplasmic

Molecular Weight: 36kDa

Gene ID: 5111, 147433, 728886

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy