PD-1 antibody

Catalog No. ABIN6938133

The Rat Monoclonal anti-PD-1 antibody is suitable to detect PD-1 in samples from Mouse. It has been validated for FACS, ELISA and IF.

Quick Overview for PD-1 antibody (ABIN6938133)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This PD-1 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Clone: RMP1-14

Product Details anti-PD-1 Antibody

Purpose: PD1, Mouse Antibody

Specificity: PD-1 is expressed on a subset of CD4-CD8-thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B-lymphocytes and myeloid cells. Additionally,Pdcd1mRNA is expressed in developing B-lymphocytes during the pro-B-cell stage. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production co-stimulated by macrophages (but not by dendritic cells and B cells).

No Cross-Reactivity: Human

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: PDCD1 transfected BHK cells

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: Spleen, Thymus or Lymph Node.

Known Application: ELISA (For coating, order Ab without BSA & Azide), Neutralization Studies (Order Ab without BSA & Azide), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PDCD1

Background:

CD279, CD279 antigen, PD1, PDCD1, Programmed cell death 1, Programmed cell death protein 1, Systemic lupus erythematosus susceptibility 2 (SLEB2)

Spleen, Thymus or Lymph Node.

Molecular Weight: ~50-55kDa

Gene ID: 18566, 5024

UniProt: Q02242

Pathways: Cancer Immune Checkpoints