Prohibitin antibody (AA 167-261)

Catalog No. ABIN6938176

The Mouse Monoclonal anti-Prohibitin antibody (Clone PHB-3194) (ABIN6938176) specifically detects Prohibitin in WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for Prohibitin antibody (AA 167-261) (ABIN6938176)

Target: Prohibitin (PHB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: PHB-3194

Product Details anti-Prohibitin Antibody

Binding Specificity: AA 167-261

Purpose: Prohibitin (Mitochondrial Marker) Antibody

Specificity: Recognizes a protein of 30 kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human PHB protein fragment (aa167-261) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Ramos, Jurkat, HEK293, K562, HepG2, A431 or MCF-7 cells (IF). Liver, Kidney, Heart or Endometrium (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: PHB

Background:

Epididymis luminal protein 215, Epididymis secretory sperm binding protein Li 54e, HEL 215, HEL S 54e, PHB, PHB1, Prohibitin

A431 or MCF-7 cells (IF). Liver, Heart or Endometrium (IHC)., HEK293, HePG2, Jurkat, K562, Kidney, Ramos

Molecular Weight: 30kDa

Gene ID: 5245, 514303

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling