Perforin 1 antibody (AA 413-552)

Catalog No. ABIN6938232

The Mouse Monoclonal anti-Perforin 1 antibody has been validated for IHC, ELISA and Coat. It is suitable to detect Perforin 1 in samples from Human.

Quick Overview for Perforin 1 antibody (AA 413-552) (ABIN6938232)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Coating (Coat)

Clone: SPM434

Product Details anti-Perforin 1 Antibody

Binding Specificity: AA 413-552

Specificity: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Perforin protein fragment (around aa 413-552) (exact sequence is proprietary)

Isotype: IgG kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA), Optimal dilution for a specific application should be determined.

Positive_Control: HDLM-2 cells. Human spleen tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: PRF1

Background: Cytolysin, FLH2, HPLH2, Lymphocyte pore-forming protein, PRF1 (pore forming protein 1), Perforin-1, PFP, PGFL, PIGF, PIGF-2, PLGF,Perforin (Pore Forming Protein) (Apoptosis Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 75kDa

Gene ID: 5551, 2200

UniProt: P14222

Pathways: Apoptosis, Caspase Cascade in Apoptosis