RBP4 antibody

Catalog No. ABIN6938313

The Mouse Monoclonal anti-RBP4 antibody has been validated for WB, IF and IHC (f). It is suitable to detect RBP4 in samples from Human.

Quick Overview for RBP4 antibody (ABIN6938313)

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RBP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SPM442

Product Details anti-RBP4 Antibody

Specificity: Recognizes a protein of 21 kDa-25 kDa, identified as retinol binding protein (RBP). Its epitope localizes between aa 74-182 of human RBP. This MAb recognizes reduced and carboxy-methylated RBP (RCM-RBP) as well as the circulatory RBP but not the native RBP, thereby suggesting that its epitope becomes accessible either on unfolding or upon binding of RBP to transthyretin (prealbumin). RBP is responsible for distributing retinol from the retinoid stores in the liver to the various target tissues. Once secreted into the blood with bound retinol, the vitamin carrier circulates complexed with transthyretin prior to vitamin delivery at the plasma membrane through a receptor-mediated mechanism.

Cross-Reactivity (Details): Human. Monkey. Goat. Rabbit. Rat. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Retinol binding protein Purified from human plasma

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Liver or placenta.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for RBP4

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Alternative Name: RBP

Background: Cellular retinol-binding protein I, CRBP1, CRBP2, RBP1, RBP2, RBP4, RBPC, Retinol binding protein 1, Retinol binding protein 1 cellular, Retinol binding protein 2 cellular, Retinol binding protein 4 plasma,Retinol Binding Protein (RBP)
Cellular localisation: Cytoplasmic

Molecular Weight: 21-25kDa

Gene ID: 5947, 5948, 5950

UniProt: P02753, P09455, P50120

Pathways: Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response