GLUT1 antibody (AA 203-305)

Catalog No. ABIN6938395

Product Details anti-GLUT1 Antibody

Target: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Binding Specificity: AA 203-305

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLUT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Recognizes a protein of 55 kDa, which is identified as GLUT-1. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut-1 to Glut-12. Glut-1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut-1 is expressed at variable levels in many human tissues. Overexpression of Glut-1 has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut-1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative).

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human GLUT1 protein (around aa 203-305) (exact sequence is proprietary)

Clone: GLUT1-2475

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating use Ab at 1-2 μg/mL order Ab without BSA), Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K562, A431, MDA-MB-231 cells. Erythrocytes. Mesothelioma or breast, colon and ovarian carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GLUT1

Target: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Alternative Name: SLC2A1 (SLC2A1 Products)

Background: Erythrocyte/hepatoma glucose transporter, Glucose transporter type-1, GLUT1, GLUT1DS, GLUTB, GT1, GTG1, Gtg3, HepG2 glucose transporter, PED, RATGTG1, Solute carrier family 2, Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1),GLUT-1 (Tumor Progression and Mesothelioma Marker)
Cellular localisation: Cell Surface

Molecular Weight: 55kDa

Gene ID: 6513, 473721

UniProt: P11166

Pathways: Sensory Perception of Sound, Dicarboxylic Acid Transport, Warburg Effect