SOX2 antibody (AA 176-305)

Catalog No. ABIN6938410

The Mouse Monoclonal anti-SOX2 antibody (Clone SOX2-1791) (ABIN6938410) specifically detects SOX2 in WB, IHC and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for SOX2 antibody (AA 176-305) (ABIN6938410)

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: SOX2-1791

Product Details anti-SOX2 Antibody

Binding Specificity: AA 176-305

Purpose: SOX2 (Embryonic Stem CellMarker) Antibody

Specificity: SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Cross-Reactivity (Details): Human. Mouse.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A549, NCCIT cells. Lung or Cervicalcarcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SOX2

Background:

ANOP3, Delta EF2a,MCOPS3 (Microphthalmia Syndromic type 3),SOX-2, SRY (sex determining region Y) box 2, SRY related HMG box 2, Transcription factor SOX-2, ysb

A549, NCCIT cells. Lung or Cervicalcarcinoma.

Molecular Weight: 34kDa

Gene ID: 6657, 518438

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis