Recombinant SOX2 antibody (AA 176-305)
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- Target See all SOX2 Antibodies
- SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))
- Antibody Type
- Recombinant Antibody
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Binding Specificity
- AA 176-305
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This SOX2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.
- Cross-Reactivity (Details)
- Human. Mouse. Dog
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)
- Clone
- SOX2-3169R
- Isotype
- IgG
- Top Product
- Discover our top product SOX2 Primary Antibody
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- Application Notes
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: A549, NCCIT cells. Lung or Cervical carcinoma.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))
- Alternative Name
- SOX2 (SOX2 Products)
- Synonyms
- ANOP3 antibody, MCOPS3 antibody, RGD1565646 antibody, Sox-2 antibody, lcc antibody, ysb antibody, cb236 antibody, wu:fb83g04 antibody, wu:fc14d07 antibody, zgc:65860 antibody, zgc:77389 antibody, SRY-box2 antibody, Xsox-2 antibody, anop3 antibody, mcops3 antibody, sox-2 antibody, SRY-box 2 antibody, SRY box 2 antibody, SRY (sex determining region Y)-box 2 antibody, SRY-box 2 S homeolog antibody, SOX2 antibody, Sox2 antibody, sox2 antibody, sox2.S antibody
- Background
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ANOP3, Delta EF2a, MCOPS3 (Microphthalmia Syndromic type 3), SOX-2, SRY (sex determining region Y) box 2, SRY related HMG box 2, Transcription factor SOX-2, ysb,SOX2 (Embryonic Stem Cell Marker)
Cellular localisation: Nuclear - Molecular Weight
- 34kDa
- Gene ID
- 6657, 518438
- UniProt
- P48431
- Pathways
- Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis
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