Spastin antibody

Catalog No. ABIN6938422

Product Details anti-Spastin Antibody

Target: Spastin (SPAST)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Spastin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40 % of all autosomal dominant HSPs.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Spastin protein.

Clone: Sp 3G11-1

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Rat brain extract.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Spastin

Target: Spastin (SPAST)

Alternative Name: SPAST (SPAST Products)

Synonyms: CG5977 antibody, D-spastin antibody, Dmel\\CG5977 antibody, Dspastin antibody, Spas antibody, zgc:85952 antibody, SPAST antibody, spg4 antibody, LOC100224472 antibody, ADPSP antibody, FSP2 antibody, SPG4 antibody, Spg4 antibody, mKIAA1083 antibody, spastin antibody, spastin S homeolog antibody, spas antibody, spast antibody, SPAST antibody, Spast antibody, spast.S antibody

Background: ADPSP, FSP2, Spast, Spatin, SPG4,Spastin
Cellular localisation: Cell Membrane and Secreted.

Molecular Weight: 52kDa

Gene ID: 6683, 468091

Pathways: Microtubule Dynamics, M Phase, Regulation of Cell Size