SREBF1 antibody

Catalog No. ABIN6938452

The Mouse Monoclonal anti-SREBF1 antibody (Clone SREBP1-1578) (ABIN6938452) specifically detects SREBF1 in IHC. The antibody is reactive with Human samples.

Quick Overview for SREBF1 antibody (ABIN6938452)

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SREBF1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: SREBP1-1578

Product Details anti-SREBF1 Antibody

Purpose: SREBP1 (Transcription Factor) Antibody

Specificity: The low density lipoprotein (LDL) receptor mediates the endocytic uptake of cholesterol-carrying lipoproteins, thereby controlling cholesterol levels in cells and plasma. Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5' flanking region, designated sterol regulatory element 1 (SRE-1). When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. A set of SREbinding proteins (SREBPs) have been identified, including two basic helixloop-helix-leucine zipper (bHLH-zip) transcription factors, designated SREBP-1 and SREBP-2. SREBP-1 and SREBP-2 have been shown to have the same specificity for SRE-1 in vitro and to activate the transcription of reporter genes containing SRE-1 in the same way.

Cross-Reactivity (Details): Human. Others not known.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human SREBP1 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Ubiqutious expression in adult and fetal tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SREBF1

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Alternative Name: SREBF1

Background:

ADD 1, bHLHd1, Class D basic helix-loop-helix protein 1, Processed sterol regulatory element-binding protein 1, SREBF1, SREBP1c, SREBP-1, Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1, Sterol regulatory element binding transcription factor 1, Sterol regulatory element-binding transcription factor 1

Ubiquitous expression in adult and fetal tissues.

Molecular Weight: 68/125kDa mature/precursor

Gene ID: 6720, 592123

UniProt: P36956

Pathways: AMPK Signaling, Caspase Cascade in Apoptosis, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha