TNC antibody

Catalog No. ABIN6938599

The Mouse Monoclonal anti-TNC antibody (Clone SPM319) (ABIN6938599) specifically detects TNC in IHC, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for TNC antibody (ABIN6938599)

Target: TNC (Tenascin C (TNC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNC antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: SPM319

Product Details anti-TNC Antibody

Purpose: Tenascin C (Stromal Marker for Epithelial Malignancy) Antibody

Specificity: In Western blotting, it reacts with two bands of ~MW of 210 kDa and 300 kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

No Cross-Reactivity: Rat (Rattus)

Cross-Reactivity (Details): Human. Does not react with Rat.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human breast carcinoma

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) ,Optimal dilution for a specific application should be determined.

Positive_Control: Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell and lung carcinomas. Staining of normal fibroblasts serves as internal positive control.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TNC

Target: TNC (Tenascin C (TNC))

Alternative Name: TNC

Background:

Cytotactin, Glioma-associated-extracellular matrix antigen, GMEM, GP 150-225, Hexabrachion (HXB), JI, Myotendinous antigen, Neuronectin, TNC

Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell and lung carcinomas. Staining of normal fibroblasts serves as internal positive control.

Molecular Weight: 210kDa and 300kDa

Gene ID: 3371, 143250

UniProt: P24821

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development