TRIM29 antibody (AA 1-200)

Catalog No. ABIN6938675

This Mouse Monoclonal antibody specifically detects TRIM29 in ELISA, IHC (f) and Coat. It exhibits reactivity toward Human.

Quick Overview for TRIM29 antibody (AA 1-200) (ABIN6938675)

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIM29 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Clone: TRIM29-1041

Product Details anti-TRIM29 Antibody

Binding Specificity: AA 1-200

Specificity: It recognizes a 66 kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90 % positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (126 Amino acid residues between aa 1-200) of human TRIM29 protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil or squamous cell carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TRIM29

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Alternative Name: TRIM29

Background: Ataxia telangiectasia group D complementing gene (ATDC), Tripartite motif-containing protein 29 (TRIM29),TRIM29 (Lung Squamous Cell Carcinoma Marker)
Cellular localisation: Cytoplasmic and Cell Surface

Molecular Weight: 66kDa

Gene ID: 23650, 504115

UniProt: Q14134

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus