TYMS antibody (AA 60-174)

Catalog No. ABIN6938693

This Mouse Monoclonal antibody specifically detects TYMS in WB, IF, FACS, IHC (f) and RIA. It exhibits reactivity toward Human.

Quick Overview for TYMS antibody (AA 60-174) (ABIN6938693)

Target: TYMS (Thymidylate Synthetase (TYMS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYMS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Radioimmunoassay (RIA)

Clone: TYMS-1884

Product Details anti-TYMS Antibody

Binding Specificity: AA 60-174

Specificity: It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)

Isotype: IgG2c kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: TYMS

Background: DTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase,Thymidylate Synthase (5-FU Resistance Marker)
Cellular localisation: Nuclear & Cytoplasmic

Molecular Weight: 36kDa

Gene ID: 7298, 369762

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases