Tyrosinase-Related Protein 1 antibody (AA 257-377)

Catalog No. ABIN6938703

Product Details anti-Tyrosinase-Related Protein 1 Antibody

Target: Tyrosinase-Related Protein 1 (TYRP1)

Binding Specificity: AA 257-377

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tyrosinase-Related Protein 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Specificity: It reacts with a 75 kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi.Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment (around aa 257-377) of human TYRP1 protein (exact sequence is proprietary)

Clone: TYRP1-3280

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: SK-MEL-23, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells. Human skin or melanoma tissues (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Tyrosinase-Related Protein 1

Target: Tyrosinase-Related Protein 1 (TYRP1)

Alternative Name: TYRP1 (TYRP1 Products)

Background: 5, 6 dihydroxyindole 2 carboxylic acid oxidase, 6-dihydroxyindole-2-carboxylic acid oxidase, Associated with iris pigmentation, CAS2, Catalase B (CATB), DHICA oxidase, Glycoprotein75 (GP75), Melanoma antigen gp75,Tyrosinase-Related Protein-1 (TYRP-1) (Melanoma Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 75kDa

Gene ID: 7306, 270279

UniProt: P17643