UCHL1 antibody

Catalog No. ABIN6938721

The Mouse Monoclonal anti-UCHL1 antibody has been validated for WB and IF. It is suitable to detect UCHL1 in samples from Human.

Quick Overview for UCHL1 antibody (ABIN6938721)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 13C4

Product Details anti-UCHL1 Antibody

Specificity: This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.

Cross-Reactivity (Details): Human. Cow. Dog. Guinea Pig. Mouse. Pig. Rabbit. Rat. Sheep. Zebrafish.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Native UchL1 (PGP9.5) protein from brain

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: Cerebellum.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: UCHL1

Background: Gracile Axonal Dystrophy, Neuron Cytoplasmic Protein 9.5, Park5, Parkinson Disease 5, PGP95, Protein Gene Product 9.5, Ubiquitin Carboxyl-terminal Esterase L1, Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, Ubiquitin Thioesterase L1, Ubiquitin Thiolesterase L1,PGP9.5 / UchL1 (pan-Neuronal Marker)
Cellular localisation: Cytoplasmic. Endoplasmic Reticulum membrane

Molecular Weight: 20-30kDa

Gene ID: 7345, 518731

UniProt: P09936

Pathways: Feeding Behaviour