VTCN1 antibody

Catalog No. ABIN6938766

Product Details anti-VTCN1 Antibody

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VTCN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment of human B7-H4 protein (exact sequence is proprietary)

Clone: B7H4-1788

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Flow Cytometry (1-2 μg/million cells in 0.1ml), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, SKBr-3 or MCF-7 cells. Human ovary, pancreas, placenta or spleen.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for VTCN1

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Alternative Name: VTCN1 (VTCN1 Products)

Synonyms: B7h4 antibody, B7s1 antibody, B7x antibody, BC032925 antibody, B7-H4 antibody, B7H4 antibody, B7S1 antibody, B7X antibody, B7h.5 antibody, PRO1291 antibody, VCTN1 antibody, RGD1311204 antibody, V set domain containing T cell activation inhibitor 1 L homeolog antibody, V-set domain containing T cell activation inhibitor 1 antibody, V-set domain containing T-cell activation inhibitor 1 antibody, V set domain containing T cell activation inhibitor 1 antibody, vtcn1.L antibody, VTCN1 antibody, vtcn1 antibody, Vtcn1 antibody

Background: B7 family member, H4, B7 homolog 4, B7 superfamily member 1, B7-H4, B7S1, Immune costimulatory protein B7-H4, T-cell costimulatory molecule B7x, V-set domain-containing T-cell activation inhibitor 1, VCTN1,B7-H4 (Immuno-Inhibitory Protein)
Cellular localisation: Cell Surface and Cytoplasmic

Molecular Weight: 35kDa

Gene ID: 79679, 546434

UniProt: Q7Z7D3