VWF antibody (AA 845-949)

Catalog No. ABIN6938770

The Mouse Monoclonal anti-VWF antibody (Clone 3E2D10) (ABIN6938770) specifically detects VWF in IHC, WB, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for VWF antibody (AA 845-949) (ABIN6938770)

Target: VWF (Von Willebrand Factor (VWF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VWF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 3E2D10

Product Details anti-VWF Antibody

Binding Specificity: AA 845-949

Purpose: von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Antibody

Specificity: Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human vWF fragment spanning around aa 845-949 (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein lysate),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HUVEC cells. Tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for VWF

Target: VWF (Von Willebrand Factor (VWF))

Alternative Name: VWF

Background:

Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

HUVEC cells. Human tonsil.

Molecular Weight: 250kDa

Gene ID: 7450, 440848

UniProt: P04275