Occludin antibody (AA 282-415)

Catalog No. ABIN6939017

This Mouse Monoclonal antibody specifically detects Occludin in WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Occludin antibody (AA 282-415) (ABIN6939017)

Target: Occludin (OCLN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: OCLN-2181

Product Details anti-Occludin Antibody

Binding Specificity: AA 282-415

Specificity: Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue, in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB). Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption.

Cross-Reactivity (Details): Human,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes:

Positive Control: HepG2 cells. Kidney.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: OCLN

Background: BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PTORCH1, Tight junction protein occludin,Occludin (Marker of Early Blood Brain Barrier Damage)
Cellular localisation: Cell Surface and Cytoplasmic

Molecular Weight: 60-82kDa

Gene ID: 100506658, 592605

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C