CDK2 antibody

Catalog No. ABIN6939030

This Mouse Monoclonal antibody specifically detects CDK2 in WB, IP, FACS, Act and KAA. It exhibits reactivity toward Human.

Quick Overview for CDK2 antibody (ABIN6939030)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Activation (Act), Kinase Activity Assay (KAA)

Clone: AN4-3

Product Details anti-CDK2 Antibody

Specificity: In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclindependent kinase 2 (Cdk2), also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2-related cyclin dependent kinases have been identified. These include Cdk3, Cdk4, Cdk5, PCTAIRE-1, PCTAIRE-2, PCTAIRE-3, Cdk6 Cdk7, Cdk8 and KKIALRE.

Cross-Reactivity (Details): Human, Mouse, Xenopus,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Cdk2 protein

Isotype: IgG2a

Application Details

Application Notes:

Positive Control: HeLa cells. HeLa whole cell lysate.

Known Application: Inhibits activation of p34cdc2 kinase by cyclins, Immunoprecipitation, Kinase Assay, ,Western Blot, Flow cytometry (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2

Background: Cdc2 related protein kinase, CDC28, CDC2A, CDK1, CDK2, CDKN2, Cell devision kinase 2, Cell division protein kinase 2, cyclin dependent kinase 2 alpha, kinase Cdc2, MPF, p33 protein kinase,Cdk2 / p34cdc2 Serine-Threonine Kinase
Cellular localisation: Nuclear

Molecular Weight: 34kDa

Gene ID: 1017, 19192

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA