MERTK antibody (AA 55-148)

Catalog No. ABIN6939061

This Mouse Monoclonal antibody specifically detects MERTK in IHC. It exhibits reactivity toward Human.

Quick Overview for MERTK antibody (AA 55-148) (ABIN6939061)

Target: MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MERTK antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MERTK-3015

Product Details anti-MERTK Antibody

Binding Specificity: AA 55-148

Purpose: MerTK (Innate Immune Checkpoint) Antibody

Specificity: MerTK, also called c-Mer, is a member of the Mer/Axl/Tyro3 receptor kinase family. It is a 984 residue transmembrane protein made up of one tyrosine kinase domain, two Fibronectin type-III domains and two immunoglobulinlike C2-type domains. MerTK is the mammalian ortholog of the chicken retroviral oncogene product v-Eyk. This protein plays a critical role in macrophage activation, platelet aggregation, clot stability and the efficient removal of apoptotic cells. Specifically, MerTK acts as a signaling molecule, triggering outer segment ingestion in the retinal pigment epithelium (RPE) phagocytic process. Evidence suggests that MerTK signals via interaction with phosphatidylinositol-specific phospholipase C Á2 (PI-PLC Á2). When the gene encoding for MerTK is mutated, the RPE phagocytosis pathway is disrupted and autosomal recessive retinitis pigmentosa (RP) may result, leading to degeneration of retinal photoreceptor cells.

Cross-Reactivity (Details): Human,

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human MERTK protein fragment (around aa 55-148) (exact sequence is proprietary)

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: Human colon tissue (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MERTK

Target: MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))

Alternative Name: MERTK

Background:

cEyk, MER receptor tyrosine kinase, MERK, MERPEN, nmf12, Nyk, Proto-oncogene c-Mer, RP38, STK kinase

Human colon tissue (IHC).

Molecular Weight: 110kDa

Gene ID: 10461, 306178

UniProt: Q12866

Pathways: RTK Signaling